Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay

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Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay

El diagnóstico de laboratorio de brucelosis es generalmente realizado por métodos microbiológicos y serológicos. La prueba PCR es reconocida como una alternativa específica y sensible para la detección de diferentes agentes bacterianos. Se realizó una evaluación de la prueba PCR para la detección de ADN de Brucella melitensis en leche de oveja. Ciento dos muestras tomadas de ovejas postaborto f...

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Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay# Detección de ADN de Brucella melitensis mediante la prueba de PCR en muestras de leche de ovejas postaborto

El diagnóstico de laboratorio de brucelosis es generalmente realizado por métodos microbiológicos y serológicos. La prueba PCR es reconocida como una alternativa específica y sensible para la detección de diferentes agentes bacterianos. Se realizó una evaluación de la prueba PCR para la detección de ADN de Brucella melitensis en leche de oveja. Ciento dos muestras tomadas de ovejas postaborto f...

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Development of a Differential PCR Assay for Detection of Brucella abortus and Brucella melitensis: an Analytical Approach for Monitoring of Brucella spp. in Foods of Animal Origin

Background: Classical bacteriological detection of Brucella species from food, and environment is routinely carried out based on morphological and biochemical characteristics. However, for increasing specificity and sensitivity of species identification methods, development of a molecular assay is necessary that was main aim of this study. Methods: Panel of some reference strains belonging to ...

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Detection of Brucella melitensis in bovine milk and milk products from apparently healthy animals in Egypt by real-time PCR.

INTRODUCTION Brucellosis in Egypt is an endemic disease among animals and humans. In endemic developing countries, dairy products produced from untreated milk are a potential threat to public health. The aim of this study was to detect brucellae in milk and milk products produced from apparently healthy animals to estimate the prevalence of contamination. METHODOLOGY Two hundred and fifteen u...

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ژورنال

عنوان ژورنال: Archivos de medicina veterinaria

سال: 2008

ISSN: 0301-732X

DOI: 10.4067/s0301-732x2008000200005